Protein microarray
Avantajele platformei: cantitate uriasa de date de la o cantitate minima de material biologic de analizat; analiza simultana de analiti multipli; datele cantitative sunt furnizate pe baza unor curbe calibrate de referinta pentru biomoleculele analizate.
Exemple de aplicatii biomedicale: semnalizare celulara in scopul studierii functiilor si interactiilor proteice din diferite procese celulare; in domeniul biomarkerilor pentru descoperirea si validarea biomarkerilor in special in cancer; screening de agenti terapeutici sau analiza efectului unor produsi asupra expresiei proteice, in scopul descoperirii de noi candidati terapeutici prin studierea interactiilor dintre medicament si proteinele implicate in procesul patologic investigat; diagnostic, unde protein array are un mare potential pentru diagnosticul in vitro, mai ales in diagnosticul medical al alergenilor sau in industria alimentara.
Echipament: scanner Innoscan 1100L – cell and tissue array
Echipament: scanner ArrayIT
Instruire Toulouse-Carbonne: la producator (Innopsys, Dr. Laurence Bouet, Dr. Adriana Lagraulet) si la un utilizator de platforme Innopsys (Universitatea din Toulouse, Fac. Biotehnologie si Nanotehnologii, Dr. Veronique La Berre)
Rezultate – protein microarray
Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy, Clara Matei, Mircea Tampa, Constantin Caruntu, Rodica-Mariana Ion, Simona-Roxana Georgescu, Georgiana Roxana Dumitrascu, Carolina Constantin and Monica Neagu, Biol Res, 2014, 47(1): 33-41
Microscopia confocala de reflectanta (RCM) - Centrul de excelenta in Dermato-oncologie
Tehnica imagistica non-invaziva, recent introduse in practica dermatologica, permite caracterizarea in vivo a pielii la rezolutie aproape histologică.
Aplicatiile tehnicii RCM:Investigarea deteriorarii cutanate datorate varstei sau/si expuneriisolare; Evaluarea pigmentarii cutanate si a terapiei asociate; Masurarea grosimii epidermale; Investigarea dermatitelor si a terapiei associate; Investigarea re-generarii cutanate si a eficientei terapiei; Investigarea aparitiei celulitei si a terapiilor anti-celulita; Investigarea scalpului si a parului si a terapiilor specifice acestora
Microscopie confocala de reflectanta - VivaScope 1500
(Figura reprodusa din articolul The role of confocal microscopy in the dermato-oncology practice. Diaconeasa A, Boda D, Neagu M, Constantin C, Căruntu C, Vlădău L, Guţu D J Med Life. 2011 Jan-Mar;4(1):63-74.)
Rezultate - Microscopia confocala in vivo
Reflectance confocal microscopy and dermoscopy for in vivo, noninvasive skin imaging of superficial basal cell carcinoma, Mihaela A Ghita, Constantin Caruntu, Adrian E Rosca, Harillaq Kaleshi, Ana Caruntu, Liliana Moraru, Sabina Zurac, Daniel Boda, Monica Neagu, Demetrios A. Spandidos, Aristidis M. Tsatsakis, Oncology Lett, March 18, 2016 DOI: 10.3892/ol.2016.4354. Wound Repair - Up-Dates in Dressing Patents and Regeneration Biomarkers, Carolina Constantin, Georgeta Paunica-Panea, Vlad D. Constantin and Monica Neagu, Recent Patents on Biomarkers, 2014, 4(3): 133-149. Căruntu C, Boda D, Guţu DE and Căruntu A: In vivo reflectance confocal microscopy of basal cell carcinoma with cystic degeneration. Rom J Morphol Embryol 55: 1437‑1441, 2014. Căruntu C, Boda D, Căruntu A, Rotaru M, Baderca F and Zurac S: In vivo imaging techniques for psoriatic lesions. Rom J Morphol Embryol 55 (Suppl 3): 1191‑1196, 2014. Căruntu C and Boda D: Evaluation through in vivo reflectance confocal microscopy of the cutaneous neurogenic inflammatory reaction induced by capsaicin in human subjects. J Biomed Opt 17: 085003, 2012. The role of confocal microscopy in the dermato-oncology practice. Diaconeasa A, Boda D, Neagu M, Constantin C, Căruntu C, Vlădău L, Guţu D J Med Life. 2011 Jan-Mar;4(1):63-74. Epub 2011 Feb 25.